Negamycin binds to the wall of the nascent chain exit tunnel of the 50S ribosomal subunit.
نویسندگان
چکیده
Negamycin, a small-molecule inhibitor of protein synthesis, binds the Haloarcula marismortui 50S ribosomal subunit at a single site formed by highly conserved RNA nucleotides near the cytosolic end of the nascent chain exit tunnel. The mechanism of antibiotic action and the function of this unexplored tunnel region remain intriguingly elusive.
منابع مشابه
The signal recognition particle binds to protein L23 at the peptide exit of the Escherichia coli ribosome.
The signal recognition particle (SRP) from Escherichia coli, composed of Ffh protein and 4.5S RNA, mediates membrane targeting of translating ribosomes displaying a signal or signal-anchor sequence. SRP binds at the peptide exit of the large ribosomal subunit. Structural details of the interaction are not known. Here, the position of Ffh or SRP on the ribosome was probed by using site-specific ...
متن کاملEnhanced SnapShot: Antibiotic Inhibition of Protein Synthesis II
The translational apparatus is one of the major targets for antibiotics in the bacterial cell. Antibiotics predominantly interact with the functional centers of the ribosome, namely the messenger RNA (mRNA)-transfer RNA (tRNA) decoding region on the 30S subunit, the peptidyltransferase center on the 50S subunit, or the ribosomal exit tunnel through which the nascent polypeptide chain passes dur...
متن کاملNMR and PXRD Analysis of the Structure of a New Hydrous Layer Silicate
C100 emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the tunnel opening and forms a sheltered folding space. The 3.5Å crystal structure of the large ribosomal subunit of the eubacterium Deinococcus radiodurans, D50S, in complex with the TF binding domain (TFa) from Deinococcus radiodurans, reveals for the first time the molecular structure of the entire TFa bound...
متن کاملStructure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone action.
Trigger factor (TF), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. Here, we present the 3.5-A crystal structure of the physiological complex of the large ribosomal subunit from the eubacterium Deinococcus radiodurans with the N-terminal domain...
متن کاملMacrolide-Peptide Conjugates as Probes of the Path of Travel of the Nascent Peptides through the Ribosome
Despite decades of research on the bacterial ribosome, the ribosomal exit tunnel is still poorly understood. Although it has been suggested that the exit tunnel is simply a convenient route of egress for the nascent chain, specific protein sequences serve to slow the rate of translation, suggesting some degree of interaction between the nascent peptide chain and the exit tunnel. To understand h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 51 12 شماره
صفحات -
تاریخ انتشار 2007